macs buffer (Miltenyi Biotec)
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Miltenyi Biotec
macs buffer
Macs Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macs+separator/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/pm42880242-236-106-108
Average 97 stars, based on 378 article reviews
Macs Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macs+separator/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/pm42880242-236-106-108
Average 97 stars, based on 378 article reviews
macs buffer - by Bioz Stars,
2026-11
97/100 stars
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other:Article Title: Integrating single-cell and spatial transcriptomics to reveal the spatiotemporal dynamics of retinal cells during ischemia-reperfusion injury progression in rats. Article Snippet: 613 Actinomycin D (Sigma-Aldrich, St. Louis, MO, USA; Cat# A1410) was added at three steps during 614 the dissociation process: 45 mM to the papain solution, 45 mM to the papain inhibitor solution, and 615 3 mM during trituration. Centrifugation:Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a Isolation:Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a Magnetic Cell Separation:Article Title: Viral Microglia Reprogramming Clears Oligomeric Neurotoxic Debris Article Snippet: The pellet was resuspended in MACS Buffer (1 x PBS, 1 mM EDTA, 1% BSA) containing CD11b + MicroBeads (Miltenyi Biotec #130-093-634; 10 μl beads per 90 μl buffer) and incubated at 4°C (15 min). .. After rinsing cells with centrifugation at 300 x g (10 min, 4°C), CD11b + cells were isolated via LS magnetic columns (Miltenyi #130-042-401) mounted on a Article Title: Microglial TDP-43 mediates myelin refinement and represses Tyrobp cryptic exon inclusion in mice. Article Snippet: Before blocking, the total amount of protein per lane was determined using REVERT Total Protein Stain Solution (LI-COR Biosciences, 926-11011) and used for normalization. .. Samples were resuspended in 1 ml and passed through an LS column (Miltenyi Biotec, 130-042-401) attached to a Article Title: Targeting TPO/MPL Signaling to Mitigate JAK2V617F-driven Cardiac Microvascular Disease Article Snippet: After washing, cell pellets were resuspended in 100ul of Dead Cell Removal Microbeads per 10 7 total cells (Cat. 130-090-101, Miltenyi Biotec) and incubated 15 minutes at room temperature. .. The mixture was diluted in 1x binding buffer to a minimum volume of 500ul and applied to a Large cell column (Cat. 130-042-202, Miltenyi Biotec) mounted on a Article Title: An improved method for high-purity isolation of oligodendrocytes from neonatal and adult mouse brain Article Snippet: Cells were then incubated with 3 μL anti-O4 microBeads (Miltenyi Biotec, 130-094-543) for 15 min under the same conditions, washed with 2 mL DPBS/BSA buffer, and centrifuged at 300 × g for 10 min. .. The pellet was resuspended in 500 μL DPBS/BSA buffer and applied through a 70-μm pre-separation filter (Miltenyi Biotec, 130-095-823) onto an MS column (Miltenyi Biotec, 130-042-201) in a Article Title: Temporal multi-omic profiling of immune, gut, and microbiome responses to ischemic stroke reveals convergence of host and microbial perturbations one week after brain injury Article Snippet: For a better cell sorting, the cell suspension was processed to further remove myelin debris with Myelin Removal Beads II (cat. 130-096-733, Miltenyi Biotec) following the manufacturer’s instructions. .. In brief, cell suspension was incubated with beads then loaded onto a LS Column (cat. 130-042-401, Miltenyi Biotec), which is placed in the magnetic field of a Article Title: Single-cell transcriptomics reveals anti-inflammatory effects of prednisolone on the hepatic niche in biliary atresia. Article Snippet: .. LS column (Miltenyi #130-042-401) was placed in a Article Title: Regulation of dendritic cells maturation via the Serpinb8/ITGAX axis in H. pylori-induced gastric mucosal injury. Article Snippet: Background: Helicobacter pylori (H. pylori) has evolved multiple immune evasion mechanisms that enable its persistent colonization in the gastric mucosa of approximately half of the global population.. The chronic inflammation resulting from this persistent infection is widely recognized as a major contributor to gastric carcinogenesis.. The specific goal of this study is to elucidate how H. pylori infection regulates dendritic cell (DC) maturation via the Serpinb8/ITGAX axis. Binding Assay:Article Title: Targeting TPO/MPL Signaling to Mitigate JAK2V617F-driven Cardiac Microvascular Disease Article Snippet: After washing, cell pellets were resuspended in 100ul of Dead Cell Removal Microbeads per 10 7 total cells (Cat. 130-090-101, Miltenyi Biotec) and incubated 15 minutes at room temperature. .. The mixture was diluted in 1x binding buffer to a minimum volume of 500ul and applied to a Large cell column (Cat. 130-042-202, Miltenyi Biotec) mounted on a Article Title: Single-cell transcriptomics reveals anti-inflammatory effects of prednisolone on the hepatic niche in biliary atresia. Article Snippet: .. LS column (Miltenyi #130-042-401) was placed in a Magnetic Beads:Article Title: Targeting TPO/MPL Signaling to Mitigate JAK2V617F-driven Cardiac Microvascular Disease Article Snippet: After washing, cell pellets were resuspended in 100ul of Dead Cell Removal Microbeads per 10 7 total cells (Cat. 130-090-101, Miltenyi Biotec) and incubated 15 minutes at room temperature. .. The mixture was diluted in 1x binding buffer to a minimum volume of 500ul and applied to a Large cell column (Cat. 130-042-202, Miltenyi Biotec) mounted on a Suspension:Article Title: Temporal multi-omic profiling of immune, gut, and microbiome responses to ischemic stroke reveals convergence of host and microbial perturbations one week after brain injury Article Snippet: For a better cell sorting, the cell suspension was processed to further remove myelin debris with Myelin Removal Beads II (cat. 130-096-733, Miltenyi Biotec) following the manufacturer’s instructions. .. In brief, cell suspension was incubated with beads then loaded onto a LS Column (cat. 130-042-401, Miltenyi Biotec), which is placed in the magnetic field of a Incubation:Article Title: Temporal multi-omic profiling of immune, gut, and microbiome responses to ischemic stroke reveals convergence of host and microbial perturbations one week after brain injury Article Snippet: For a better cell sorting, the cell suspension was processed to further remove myelin debris with Myelin Removal Beads II (cat. 130-096-733, Miltenyi Biotec) following the manufacturer’s instructions. .. In brief, cell suspension was incubated with beads then loaded onto a LS Column (cat. 130-042-401, Miltenyi Biotec), which is placed in the magnetic field of a Labeling:Article Title: Regulation of dendritic cells maturation via the Serpinb8/ITGAX axis in H. pylori-induced gastric mucosal injury. Article Snippet: Background: Helicobacter pylori (H. pylori) has evolved multiple immune evasion mechanisms that enable its persistent colonization in the gastric mucosa of approximately half of the global population.. The chronic inflammation resulting from this persistent infection is widely recognized as a major contributor to gastric carcinogenesis.. The specific goal of this study is to elucidate how H. pylori infection regulates dendritic cell (DC) maturation via the Serpinb8/ITGAX axis. |